Molecular and expression characterization of Toll-like receptor family genes from the Anadara sativa (Bivalvia, Arcidae) transcriptome.
Identifieur interne : 000109 ( Main/Exploration ); précédent : 000108; suivant : 000110Molecular and expression characterization of Toll-like receptor family genes from the Anadara sativa (Bivalvia, Arcidae) transcriptome.
Auteurs : Yipeng Ren [République populaire de Chine] ; Wenhao Dong [République populaire de Chine] ; Yi Yang [République populaire de Chine] ; Baoping Pan [République populaire de Chine] ; Wenjun Bu [République populaire de Chine]Source :
- Developmental and comparative immunology [ 1879-0089 ] ; 2020.
Abstract
Innate immunity plays an important role in invertebrates because it provides the first line of protection by recognizing invading microbial pathogens and then activating downstream signaling pathways. However, until now, increasing reports of clam diseases did not include those of Anadara sativa, which are widely distributed and economically important maritime clams. In the present study, transcriptome libraries of untreated (termed H) and Vibrio anguillarum-challenged (termed HV) A. sativa hepatopancreases were constructed and sequenced using the Illumina HiSeq4000 platform. In total, we obtained 78,012,510 and 84,937,516 clean reads from 80,006,030 to 86,871,742 raw data reads, respectively, assembled by different software programs. Furthermore, 150,274 unigenes were generated from 196,003 transcripts, with an N50 length of 1088 bp, and then annotated with the SwissProt, NR, NT, PFAM, KO, GO, KOG and KEGG databases. Moreover, 3982 differentially expressed unigenes (H vs HV) were determined, with 3583 upregulated and 399 downregulated genes. Among these differentially expressed unigenes, 207 unigenes were found using KEGG annotation in 16 immune-related signaling pathways, such as Toll-like receptor (TLR), NOD-like receptor (NLR), and RIG-I-like receptor (RLR) signaling pathways. Finally, we selected 11 full-length TLRs and classified them into 3 groups, namely, one V-TLR, four Ls-TLR and six sP-TLR; furthermore, we validated the increased expression patterns of the 11 TLRs in response to LPS injection. In summary, these results revealed multiple findings on potential immune-related genes, such as the differential expression analysis and annotation based on the A. sativa transcriptome in response to V. anguillarum stimulation, and explored the molecular and expression characterization of A. sativa TLRs, which provide new insights into the innate immune responses and defense mechanisms in shellfish.
DOI: 10.1016/j.dci.2020.103630
PubMed: 31981574
Affiliations:
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Electronic address: renyipeng@nankai.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071</wicri:regionArea><wicri:noRegion>300071</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Wenhao" sort="Dong, Wenhao" uniqKey="Dong W" first="Wenhao" last="Dong">Wenhao Dong</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071</wicri:regionArea><wicri:noRegion>300071</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yi" sort="Yang, Yi" uniqKey="Yang Y" first="Yi" last="Yang">Yi Yang</name><affiliation wicri:level="1"><nlm:affiliation>Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060</wicri:regionArea><wicri:noRegion>300060</wicri:noRegion></affiliation></author><author><name sortKey="Pan, Baoping" sort="Pan, Baoping" uniqKey="Pan B" first="Baoping" last="Pan">Baoping Pan</name><affiliation wicri:level="1"><nlm:affiliation>Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387</wicri:regionArea><wicri:noRegion>300387</wicri:noRegion></affiliation></author><author><name sortKey="Bu, Wenjun" sort="Bu, Wenjun" uniqKey="Bu W" first="Wenjun" last="Bu">Wenjun Bu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China. Electronic address: wenjunbu@nankai.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071</wicri:regionArea><wicri:noRegion>300071</wicri:noRegion></affiliation></author></analytic><series><title level="j">Developmental and comparative immunology</title><idno type="eISSN">1879-0089</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Innate immunity plays an important role in invertebrates because it provides the first line of protection by recognizing invading microbial pathogens and then activating downstream signaling pathways. However, until now, increasing reports of clam diseases did not include those of Anadara sativa, which are widely distributed and economically important maritime clams. In the present study, transcriptome libraries of untreated (termed H) and Vibrio anguillarum-challenged (termed HV) A. sativa hepatopancreases were constructed and sequenced using the Illumina HiSeq4000 platform. In total, we obtained 78,012,510 and 84,937,516 clean reads from 80,006,030 to 86,871,742 raw data reads, respectively, assembled by different software programs. Furthermore, 150,274 unigenes were generated from 196,003 transcripts, with an N50 length of 1088 bp, and then annotated with the SwissProt, NR, NT, PFAM, KO, GO, KOG and KEGG databases. Moreover, 3982 differentially expressed unigenes (H vs HV) were determined, with 3583 upregulated and 399 downregulated genes. Among these differentially expressed unigenes, 207 unigenes were found using KEGG annotation in 16 immune-related signaling pathways, such as Toll-like receptor (TLR), NOD-like receptor (NLR), and RIG-I-like receptor (RLR) signaling pathways. Finally, we selected 11 full-length TLRs and classified them into 3 groups, namely, one V-TLR, four Ls-TLR and six sP-TLR; furthermore, we validated the increased expression patterns of the 11 TLRs in response to LPS injection. In summary, these results revealed multiple findings on potential immune-related genes, such as the differential expression analysis and annotation based on the A. sativa transcriptome in response to V. anguillarum stimulation, and explored the molecular and expression characterization of A. sativa TLRs, which provide new insights into the innate immune responses and defense mechanisms in shellfish.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31981574</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0089</ISSN><JournalIssue CitedMedium="Internet"><Volume>106</Volume><PubDate><Year>2020</Year><Month>05</Month></PubDate></JournalIssue><Title>Developmental and comparative immunology</Title><ISOAbbreviation>Dev Comp Immunol</ISOAbbreviation></Journal><ArticleTitle>Molecular and expression characterization of Toll-like receptor family genes from the Anadara sativa (Bivalvia, Arcidae) transcriptome.</ArticleTitle><Pagination><MedlinePgn>103630</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0145-305X(19)30625-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.dci.2020.103630</ELocationID><Abstract><AbstractText>Innate immunity plays an important role in invertebrates because it provides the first line of protection by recognizing invading microbial pathogens and then activating downstream signaling pathways. However, until now, increasing reports of clam diseases did not include those of Anadara sativa, which are widely distributed and economically important maritime clams. In the present study, transcriptome libraries of untreated (termed H) and Vibrio anguillarum-challenged (termed HV) A. sativa hepatopancreases were constructed and sequenced using the Illumina HiSeq4000 platform. In total, we obtained 78,012,510 and 84,937,516 clean reads from 80,006,030 to 86,871,742 raw data reads, respectively, assembled by different software programs. Furthermore, 150,274 unigenes were generated from 196,003 transcripts, with an N50 length of 1088 bp, and then annotated with the SwissProt, NR, NT, PFAM, KO, GO, KOG and KEGG databases. Moreover, 3982 differentially expressed unigenes (H vs HV) were determined, with 3583 upregulated and 399 downregulated genes. Among these differentially expressed unigenes, 207 unigenes were found using KEGG annotation in 16 immune-related signaling pathways, such as Toll-like receptor (TLR), NOD-like receptor (NLR), and RIG-I-like receptor (RLR) signaling pathways. Finally, we selected 11 full-length TLRs and classified them into 3 groups, namely, one V-TLR, four Ls-TLR and six sP-TLR; furthermore, we validated the increased expression patterns of the 11 TLRs in response to LPS injection. In summary, these results revealed multiple findings on potential immune-related genes, such as the differential expression analysis and annotation based on the A. sativa transcriptome in response to V. anguillarum stimulation, and explored the molecular and expression characterization of A. sativa TLRs, which provide new insights into the innate immune responses and defense mechanisms in shellfish.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Yipeng</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China. Electronic address: renyipeng@nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Wenhao</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Baoping</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Tianjin Key Laboratory of Animal and Plant Resistance, School of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bu</LastName><ForeName>Wenjun</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, 300071, PR China. Electronic address: wenjunbu@nankai.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Dev Comp Immunol</MedlineTA><NlmUniqueID>7708205</NlmUniqueID><ISSNLinking>0145-305X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Anadara sativa</Keyword><Keyword MajorTopicYN="Y">Hepatopancreas</Keyword><Keyword MajorTopicYN="Y">Toll-like receptors</Keyword><Keyword MajorTopicYN="Y">Transcriptome library</Keyword><Keyword MajorTopicYN="Y">Vibrio anguillarum</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31981574</ArticleId><ArticleId IdType="pii">S0145-305X(19)30625-1</ArticleId><ArticleId IdType="doi">10.1016/j.dci.2020.103630</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ren, Yipeng" sort="Ren, Yipeng" uniqKey="Ren Y" first="Yipeng" last="Ren">Yipeng Ren</name></noRegion><name sortKey="Bu, Wenjun" sort="Bu, Wenjun" uniqKey="Bu W" first="Wenjun" last="Bu">Wenjun Bu</name><name sortKey="Dong, Wenhao" sort="Dong, Wenhao" uniqKey="Dong W" first="Wenhao" last="Dong">Wenhao Dong</name><name sortKey="Pan, Baoping" sort="Pan, Baoping" uniqKey="Pan B" first="Baoping" last="Pan">Baoping Pan</name><name sortKey="Yang, Yi" sort="Yang, Yi" uniqKey="Yang Y" first="Yi" last="Yang">Yi Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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